A specific antimicrobial protein CAP-1 from Pseudomonas sp isolated from the jellyfish Cyanea capillata

作者：Yin, Manman; Liu, Dan; Xu, Feng; Xiao, Liang; Wang, Qianqian; Wang, Beilei; Chang, Yinlong; Zheng, Jiemin; Tao, Xia; Liu, Guoyan^*; Zhang, Liming

来源：International Journal of Biological Macromolecules, 2016, 82: 488-496.

DOI：10.1016/j.ijbiomac.2015.10.056

• Antimicrobial protein
• Antimicrobial activity
• Cyanea capillata

A bacterium strain, designated as CMF-2, was isolated from the jellyfish Cyanea capillata and its culture supernatant exhibited a significant antimicrobial activity. The strain CMF-2 was identified as Pseudomonas sp. based on the morphological, biochemical and physiological characteristics as well as 165 rRNA sequence analysis. In this study, an antimicrobial protein, named as CAP-1, was isolated from the culture of CMF-2 through ammonium sulfate precipitation and gel filtration chromatography. According to the result of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), a major band indicated that the antimicrobial protein had a molecular mass of about 15 kDa, and it was identified as a hypothetical protein by MALDI-TOF-MS analysis and Mascot searching. CAP-1 displayed a broad antimicrobial spectrum against the indicator bacteria and fungus, including Staphylococcus aureus, Escherichia coli, Bacillus subtilis and Candida albicans, especially some marine-derived microorganisms such as Vibrio vulnificus, Vibrio alginolyticus, Vibrio parahaemolyticus, Vibrio cholera, and Vibrio anguillarum, but showed little impact on tumor cells and normal human cells. The protein CAP-1 remained a stable antimicrobial activity in a wide range of temperature (20-80 degrees C) and pH (2-10) conditions. These results suggested that CAP-1 might have a specific antimicrobial function not due to cytotoxicity.

• 出版日期2016-1
• 单位大连海洋大学; 天津市中医药研究院附属医院; 中国人民解放军第二军医大学