摘要

<正>Objective To study the phenotype and tumorigenicity of SHG-44 glioma stem cell spheres and the pathological characteristics of their xenograft tumors.Methods SHG-44 glioma cells were cultured under neural stem cell medium and glioma stem cell spheres were collected.Immunocytochemistry was used to dectet the expression of CD133,nestin,A2B5,vimentin,VEGFR-2 and IDH R132H.Cell spheres were induced using serum-