A novel nanosensor composed of aptamer bio-dots and gold nanoparticles for determination of thrombin with multiple signals

作者：Kuang, Lan; Cao, Shu-Ping; Zhang, Li; Li, Qiu-Hong; Liu, Zhi-Chao; Liang, Ru-Ping; Qiu, Jian-Ding^*

来源：Biosensors and Bioelectronics, 2016, 85: 798-806.

DOI：10.1016/j.bios.2016.05.096

• Bio-dots
• Aptamer
• Thrombin
• FRET
• Multiple signals

Thrombin is a crucial multifunctional enzyme involved in many physiological and pathological processes. Its detection is of great importance. In this work, a novel bio-dots nanosensor for detection of thrombin with colorimetric, fluorometric and light-scattering signals is developed. This nanosensor is composed of thrombin-binding aptamer bio-dots (TBA-dots) and gold nanoparticles (AuNP5), where TBA-dots serve as fluorometric reporter and AuNPs function as multiple roles as colorimetric reporter, light scattering reporter and fluorescence quencher. TBA-dots retain inherent structures of aptamer to specifically interact with thrombin and simultaneously induce the aggregation of AuNPs. The mechanism of the sensing system is based on distance-dependent aggregation of AuNPs and fluorescence resonance energy transfer (FRET). The nanosensor needs no further surface functionalization required for the as-prepared bio-dots and AuNP5, which provides a sensitive method for the selective detection of thrombin with a detection limit as low as 0.59 nM. In addition, it provides a brand new perspective for bio-dots and its potential use in bioanalysis.

• 出版日期2016-11-15
• 单位南昌大学