Simultaneous detection of four nitrofuran metabolites in honey using a multiplexing biochip screening assay

作者：O'Mahony John; Moloney Mary; McConnell Robert I; Benchikh El O; Lowry Philip; Furey Ambrose; Danaher Martin^*

来源：Biosensors and Bioelectronics, 2011, 26(10): 4076-4081.

DOI：10.1016/j.bios.2011.03.036

• Biochip array sensor
• Nitrofurans
• Honey

A chemiluminescence-based biochip array sensing technique has been developed and applied to the screening of honey samples for residues of banned nitrofuran antibiotics. Using a multiplex approach, metabolites of the four main nitrofuran antibiotics could be simultaneously detected. Individual antibodies specific towards the metabolites were spotted onto biochips. A competitive assay format, with chemiluminescent response, was employed. The method was validated in accordance with EU legislation (2002/657/EC, 2002), and assessed by comparison with UHPLC-MS/MS testing of 134 honey samples of worldwide origin. A similar extraction method, based on extraction of the analytes on Oasis (TM) SPE cartridges, followed by derivatisation with nitrobenzaldehyde and partition into ethyl acetate, was used for both screening and LC-MS/MS methods. The biochip array method was capable of detecting all four metabolites below the reference point for action of 1 mu g kg(-1). The detection capability was below 0.5 mu g kg(-1) for the metabolites AHD, AOZ and AMOZ; it was below 0.9 mu g kg(-1) for SEM. IC(50) values ranged from 0.14 mu g kg(-1) (AMOZ) to 2.19 mu g kg(-1) (SEM). This biosensor method possesses the potential to be a fit-for-purpose screening technique in the arena of food safety technology.

• 出版日期2011-6-15