A Triple-Emission Fluorescent Probe for Discriminatory Detection of Cysteine/Homocysteine, Glutathione/Hydrogen Sulfide, and Thiophenol in Living Cells

作者：Yang, Lei; Su, Yuanan; Geng, Yani; Zhang, Yun; Ren, Xiaojie; He, Long; Song, Xiangzhi^*

来源：ACS Sensors, 2018, 3(9): 1863-1869.

DOI：10.1021/acssensors.8b00685

• triple-emission
• fluorescent probe
• thiols
• discriminatory
• imaging

Thiols, such as cysteine (Cys), homocysteine (Hcy), glutathione (GSH), hydrogen sulfide (H2S), and thiophenol are metabolically correlated with each other via redox reactions. As a result of the similarity of chemical properties between Cys, Hcy, GSH, H2S, and thiophenol, it is very challenging to develop an effective methodology to differentiate them. In this work, a triple-emission fluorescent probe, NCQ, was reported for the simultaneous detection of Cys/Hcy, GSH/H2S, and thiophenol with high sensitivity and selectivity. The solution of NCQ displayed distinct fluorescent signals toward Cys/Hcy, GSH/H2S, and thiophenol: blue and green for Cys/Hcy, blue for GSH/H2S, blue and red for thiophenol. Through the blue-green-red emission color combination, Cys/Hcy, GSH/H2S, and thiophenol could be discriminatively detected in solution and in living cells.

• 出版日期2018-9
• 单位中南大学