科研之友
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摘要
Background: Human bone marrow-derived mesenchymal stromal cells (hMSCs) may serve as ideal delivery vehicles for gene therapy for human gliomas. The stability of hMSCs in culture had been concerned and studied, while the stability of these cells in glioma environment had been overlooked and remains unclear. In this study, we investigate the alterations of expression profile of cancer-related genes in hMSCs under the influence of glioma cells in vitro. Methods: HMSCs were obtained from normal adult persons and identified by analysis of distinct surface markers by flow cytometry and tests of their stemness, and hMSCs were co-cultured with U251 glioma cells and the expression profile of cancer-related genes were investigated by microarray assay, and the results of microarray were verified by Real-time quantitative RT-PCR. Results: The obtained hMSCs express distinct surface markers of mesenchymal stromal cells and could be induced to differentiate into neural lineage cells in vitro. Of the 440 cancer- related genes covered by Oligo GEArray Human Cancer Microarray OHS-802, 16 were found to be significantly up-regulated (> 3-fold) but none down-regulated in hMSCs co-cultured with U251 glioma cells. The up-regulations of some of those genes were confirmed by Real-time quantitative RT-PCR. The up-regulated genes include some important oncogenes. Conclusions: The present study is the first to show that the co-culture of hMSCs with human glioma cells lead to up-regulated expressions of some important oncogenes in hMSCs, the over-expression of which has been demonstrated to contribute to tumorigenesis. We propose concerns about oncological risk of hMSCs under the influence of glioma cells, and highlight the need for further studies before their clinical application as therapeutic vectors to treat human gliomas.
- 出版日期2016
- 单位广东医科大学
