Development of monoclonal antibodies to human kallikrein-related peptidase 6 (KLK6) and their use in an immunofluorometric assay for free KLK6

作者：Ott Catherine; Ainciburu Mireille; Parent Christelle; Michel Sandrine; Roesch Celine; Vourc'h Patrick; Corcia Philippe; Heuze Vourc'h Nathalie; Jolivet Reynaud Colette; Courty Yves^*

来源：Biological Chemistry, 2014, 395(9): 1119-1126.

DOI：10.1515/hsz-2014-0133

• biomarker
• ELISA
• free KLK6
• kallikrein-related peptidase

We have raised monoclonal antibodies against KLK6 and constructed a 'sandwich' type immunoassay using 8A8G3 as capture and 3H3E9 as tracer antibodies. 8A8G3 bound to one side of KLK6, whereas 3H3E9 probably bound near its catalytic site. The assay did not detect complexed forms of KLK6, indicating that it quantifies only free KLK6 (fKLK6). fKLK6 was higher in serum of patients with ovarian cancer (11.34 mu g/l +/- 1.37) than in controls (3.39 mu g/l +/- 0.42). The cerebrospinal fluid contained high concentrations of fKLK6 (257-965 mu g/l). This assay could facilitate the evaluation of fKLK6 in human diseases.

• 出版日期2014-9