Antifungal activity and expression patterns of extracellular chitinase and beta-1,3-glucanasein Wickerhamomyces anomalus EG2 treated with chitin and glucan

作者：Hong Sin Hyoung; Song Yong Su; Seo Dong Jun; Kim Kil Yong; Jung Woo Jin^*

来源：Microbial Pathogenesis, 2017, 110: 159-164.

DOI：10.1016/j.micpath.2017.06.038

• Wickerhamomyces anomalus EG2
• Chitinase
• beta-1,3-Glucanase
• Chitinase inhibitors
• Antifungal activity

In this study, the expression patterns of extracellular chitinase and beta-1,3-glucanase from cultured Wickerhamomyces anomalus EG2 treated with chitin, glucan, and chemical chitinase inhibitors (kinetin, caffeine, and acetazolamide) were investigated using SDS-PAGE. Relationship between enzyme expression and antifungal activity from yeast plays a very important role for biocontrol of phytopathoges. To determine antifungal activity against phytopathogens, W anomalus EG2 was shown to strongly inhibit hyphal growth of Fusarium oxysporum KACC 40032 and Rhizoctonia solani KACC 40111. Slight chitinase activity was observed 12 h after incubation in both PDB and YPD medium without colloidal chitin. The molecular weight of chitinase was approximately 124 kDa beta-1,3-Glucanase isoenzyme (GN1 and GN2) was observed distinctly on SDS-PAGE gels when laminarin was used as a substrate. beta-1,3-Glucanase isoenzyme was not observed when using glucan-containing high polymer complex (GHPC) as a substrate. Production of chitinase from W anomalus EG2 was inhibited slightly by acetazolamide. Abnormal and cluster-shaped cells of W anomalus EG2 were observed in both PDB and YPD medium treated with colloidal chitin. These results indicated that W anomalus EG2 could be applied commercially as a biological control agent of phytopathogens and as a bioinhibitor of yeast cell growth.

• 出版日期2017-9