科研之友
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摘要
The anaerobic process of coking wastewater treatment is the key step for the subsequent aerobic biodegradation. Understanding the bacterial community involved in anaerobic bioreactor will facilitate coking wastewater treatment optimization. In this study, the phylogenetic composition of bacterial community in the sludge of anaerobic process of coking wastewater treatment was investigated using a culture-independent molecular approach. The physico-chemical characteristics of influent and effluent coking wastewater in anaerobic process were analyzed by GC/MS methods to examine their effects on the bacterial communities. Of the 103 clones analyzed, a total of 36 operational taxonomic units (OTUs) were found. Molecular phylogenetic analysis revealed that more than 80 % of the screened clones were affiliated with Proteobacteria. The other 20 % of the total clones were either affiliated with Low-G+C gram-positive bacteria, the Cytophaga-Flexibacter-Bacteroides (CFB) group and Verrucomicrobia or Spirochaetes, where the clone distribution was 12.9 %, 4 %, 2 % and 1 %, respectively. The most frequent OTUs represented more than 40 % of the total bacterial 16S rDNA sequences, and 78 % of the OTUs comprised a single clone. Some OTUs fell into genera or taxons that were classically identified within anaerobic treatment systems and expected with known functions. The high toxic compounds within anaerobic process of coking wastewater treatment may also select a diverse bacterial population. This indicated that the bacterial communities associated with the anaerobic process of coking wastewater treatment was far more complex and variable than expected and thus could be challenging to control.
