A novel protein immobilization approach based on mercapto-poly(allylamine hydrochloride) self-assembled membrane for piezoelectric immunosensors

作者：Chen, H; Wu, ZY; Wang, H; Shen, GL^*; Yu, RQ

来源：Chinese Journal of Analytical Chemistry, 2003, 31(8): 897-900.

DOI：10.3321/j.issn:0253-3820.2003.08.001

• mercapto self-assembled membrane
• immobilization
• piezoelectric immunosensor

A novel protein immobilization approach for piezoelectric immunosensors has been proposed, which is based on the self-assembled membrane (SAM) of mercapto-poly (allylamine hydrochloride) (PAH) formed by coupling mercaptopropionic acid (MPA) to PAH via 1-ethyl-3-(3-dimethylamino-propl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS). After self-assembling a mercapto-PAH SAM on the gold electrode of the quartz crystal, the goat-anti-IgM antibody is immobilized via cross-linking with glutaraldehyde. Comparing with the MPA SAM method, this new approach shows greater frequency response and higher sensitivity. The detecting linear range of the piezoelectric immunosensor employing mercapto-PAH SAM is 0. 66 similar to 26. 4 mg/L, and that of the immunosensor employing MPA SAM is 2.2 similar to 24.2 mg/L.

• 出版日期2003-8
• 单位湖南大学; 湖南中医药大学