A novel simple satellite DNA is colocalized with the Stalker retrotransposon in Drosophila melanogaster heterochromatin

作者：Makunin IV; Pokholkova GV; Kholodilov NG; Zakharkin SO; Bonaccorsi S; Dimitri P^*; Zhimulev IF

来源：Molecular and General Genetics, 1999, 261(2): 381-387.

• Drosophila melanogaster
• constitutive heterochromatin
• satellite DNA
• transposable elements

In the T(1;2)dor( var7) multibreak rearrangement the distal 1A-2B segment of the X chromosome of; Drosophila melanogaster is juxtaposed to an inverted portion of the heterochromatin of chromosome 2. Analysis of mitotic chromosomes by a series of handing techniques has permitted us precisely to locate the heterochromatic breakpoint of this translocation in the h42 region of 2R. Cloning and sequencing of the eu-heterochromatic junction revealed that the translocated 1A-2B fragment is joined to (AACAC)(n) repeats, which represent a previously undescribed satellite DNA in D. melanogaster. These repeated sequences have been estimated to account for about 1 Mb of the D. melanogaster genome. The repeats are located mainly in the Y chromosome and in the heterochromatin of the right arm of chromosome 2 (2Rh), where they are colocalized with the Stanier retrotransposon.

• 出版日期1999-3