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摘要
This study was aimed to investigate the correlation of mast cells related microRNAs (miRNA) with risk of allergic rhinitis (AR) as well as disease severity. 85 patients with AR were recruited in this cross-sectional study, and 57 non-atopic patients with obstructive snoring undergoing adenoid surgery were also enrolled as controls. Nasal mucosa samples were collected from all participants and miRNAs were measured by Real-Time PCR methods. MicroRNA-123 (miR-126), miR-221, miR-222, miR-142-3p, miR-155, miR-132 and miR-143 were selected as candidate miRNAs base on screening of previous studies. Nasal mucosa miR-221 expression was elevated in AR patients compared to controls (1.989 (1.193-2.992) vs 1.575 (1.108-2.249), P = 0.014), as well as miR-142-3p level (3.355 (2.563-4.314) vs 1.982 (1.405-3.156), P<0.001). While no difference was found in other miRNAs between two groups. Receiver Operating Characteristic (ROC analysis) exhibited combination of miR-221 and miR-142-3p levels could predict the risk of AR with a high area under curve (AUC): 0.782, 95% CI: 0.707-0.858, as the sensitivity and specificity were 81.2% and 64.9% respectively at the best cut-off point, which is the point that has the best sensitivity and specificity. Additionally, AUC of individual miR-221 and miR-142-3p for AR were 0.622 (95% CI: 0.530-0.715) and 0.762 (95% CI: 0.683-0.841), respectively. Besides, miR-221 expression was illuminated to be positive correlated with total nasal symptom score (TNSS) (P = 0.047), itching score (P = 0.011) and sneezing score (P = 0.022). This study indicated nasal mucosa miR-221 and miR-142-3p expressions could be served as novel and promising biomarker for risk of AR, and miR-221 expression was associated with disease severity of AR.
- 出版日期2017
- 单位上海交通大学
