Ambient levels of concentrated PM2.5 affects cell kinetics in adrenal glands: an experimental study in mice

作者：Portugal Fuchs Luiz Fernando; Veras Mariana Matera; Nascimento Saldiva Paulo Hilario; da Silva Sasso Gisela Rodrigues; Carvalho Katia Candido; Simoes Manuel de Jesus; Soares Jose Maria Jr^*; Baracat Edmund Chada

来源：Gynecological Endocrinology, 2017, 33(6): 490-495.

DOI：10.1080/09513590.2017.1291617

• Adrenal
• mouse
• PM2.5
• traffic
• urban pollution

We evaluated the effects of air pollution on the adrenal cortex using 30 female mice divided into two groups of fifteen animals each. One group was conditioned daily in a chamber with exposure to particulate matter (PM) 2.5 mu m (GExp). Animals were exposed on daily basis in an ambient particles concentrator during the period of time enough to reach an accumulated dose of 600 mu g/m(3), which corresponds to a 24-h exposure of 25 mu g/m(3) that approximates to the annual mean of PM2.5 in Sao Paulo. The other group was allocated to another chamber with filtered air (GCrt). After euthanasia, the adrenals underwent histological processing and immunohistochemistry staining for Ki-67 and cleaved caspase-3. Histomorphometry of the adrenal glands in GExp showed increased thickness of the zona glomerulosa, while in GCrt; the adrenal glands from GExp had higher Ki-67 immunostaining scores in the zona reticularis than those from GCrt. The adrenal from GExp showed higher cleaved caspase-3 immunoreactivity in the zona fasciculata than the unexposed group (GCrt). The homeostasis index indicated higher cell proliferation in the zona glomerulosa and zona reticularis in GExp than in GCrt. Our data indicate that PM2.5 air pollution induces alterations on cell kinetics in mouse adrenal glands.

• 出版日期2017