Acute application of antioxidants protects against hyperoxia-induced reduction of plasma nitrite concentration

作者：Vucinovic Zoran; Duplancic Darko; Seselja Perisin Ana; Kukoc Modun Lea; Gunjaca Grgo; Radman Maja; Vukovic Jonatan; Tsikas Dimitrios; Poljak Kolja; Modun Darko^*

来源：Clinical Physiology and Functional Imaging, 2015, 35(1): 76-80.

DOI：10.1111/cpf.12169

• arterial stiffness
• nitric oxide
• oxygen
• vasoconstriction
• vitamin C

We investigated the effects of acute intake of antioxidants on hyperoxia-induced oxidative stress, reduction of plasma nitrite and change in arterial stiffness. Twelve healthy males randomly consumed either placebo or an oral antioxidant cocktail (vitamin C, 1000mg; vitamin E, 600IU; alpha-lipoic acid, 600mg). Every therapy was consumed once, a week apart, in a cross-over design, 30min before the experiment. The volunteers breathed 100% normobaric oxygen between 30th and 60th min of 1-h study protocol. Plasma levels of nitrite, lipid peroxides (LOOH) and vitamin C, arterial stiffness (indicated by augmentation index, AIx) and arterial oxygen (PtcO2) pressure were measured before and after hyperoxia. Exposure to oxygen caused a similar increase of PtcO2 in both placebo and antioxidants groups, confirming comparable exposure to hyperoxia (438 +/- 100 versus 455 +/- 83mm Hg). Vitamin C was increased in the antioxidants group confirming successful application of antioxidants (69 +/- 14 versus 57 +/- 15m). Hyperoxia resulted in increased AIx and LOOH and decreased nitrite in placebo (-32 +/- 11 versus -47 +/- 13%, 72 +/- 7 versus 62 +/- 6m H2O2 and 758 +/- 184 versus 920 +/- 191nm, respectively), but not in the antioxidants group (-42 +/- 13 versus -50 +/- 13%, 64 +/- 9 versus 61 +/- 8m H2O2 and 847 +/- 156 versus 936 +/- 201nm, respectively). The acute intake of selected antioxidants was effective in preserving bioavailabity of NO and vascular function, against hyperoxia-induced oxidative stress.

• 出版日期2015-1