Assessing Accumulated Hard-tissue Debris Using Micro-computed Tomography and Free Software for Image Processing and Analysis

作者：De Deus Gustavo^*; Marins Juliana; Neves Aline de Almeida; Reis Claudia; Fidel Sandra; Versiani Marco A; Alves Haimon; Lopes Ricardo Tadeu; Paciornik Sidnei

来源：Journal of Endodontics, 2014, 40(2): 271-276.

DOI：10.1016/j.joen.2013.07.025

• Biomechanical preparation
• debris
• free software
• irrigation
• micro-computed tomography
• root canal system

Introduction: The accumulation of debris occurs after root canal preparation procedures specifically in fins, isthmus, irregularities, and ramifications. The aim of this study was to present a step-by-step description of a new method used to longitudinally identify, measure, and 3-dimensionally map the accumulation of hard-tissue debris inside the root canal after biomechanical preparation using free software for image processing and analysis. Methods: Three mandibular molars presenting the mesial root with a large isthmus width and a type II Vertucci's canal configuration were selected and scanned. The specimens were assigned to 1 of 3 experimental approaches: (1) 5.25% sodium hypochlorite + 17% EDTA, (2) bidistilled water, and (3) no irrigation. After root canal preparation, high-resolution scans of the teeth were accomplished, and free software packages were used to register and quantify the amount of accumulated hard-tissue debris in either canal space or isthmus areas. Results: Canal preparation without irrigation resulted in 34.6% of its volume filled with hard-tissue debris, whereas the use of bidistilled water or NaOCl followed by EDTA showed a reduction in the percentage volume of debris to 16% and 11.3%, respectively. The closer the distance to the isthmus area was the larger the amount of accumulated debris regardless of the irrigating protocol used. Conclusions:,Through the present method, it was possible to calculate the volume of hard-tissue debris in the isthmuses and in the root canal space. Free-software packages used for image reconstruction, registering, and analysis have shown to be promising for end-user application.

• 出版日期2014-2