Discovery of Cryptic Polyketide Metabolites from Dermatophytes Using Heterologous Expression in Aspergillus nidulans

作者：Yin Wen Bing; Chooi Yit Heng; Smith Adam R; Cacho Ralph A; Hu Youcai; White Theodore C; Tang Yi^*

来源：ACS Synthetic Biology, 2013, 2(11): 629-634.

DOI：10.1021/sb400048b

• natural products
• polyketide
• heterologous expression
• prenyltransferase

Dermatophytes belonging to the Trichophyton and Arthroderma genera cause skin infections in humans and animals. From genome sequencing data, we mined a conserved gene cluster among dermatophytes that are homologous to one that produces an immunosuppressive polyketide in Aspergillus fumigatus. Using a recombination-based cloning strategy in yeast, we constructed fungal heterologous expression vectors that encode the cryptic clusters. When integrated into the model Aspergillus nidulans host, a structurally related compound neosartoricin B was formed, suggesting a possible role of this compound in the pathogenesis of these strains.

• 出版日期2013-11