Detection of H2O2 at Composite Membrane of [bmim] FeCl4 and PVA Loading HRP Modified Graphene/nano Au

作者：Qu Jian Ying^*; Kang Shi Ping; Lou Tong Fang; Du Xue Ping

来源：Chemical Journal of Chinese Universities, 2013, 34(9): 2097-2101.

DOI：10.7503/cjcu20121075

• Biosensor
• Nano-Au
• Graphene
• 1-Butyl-3-methylimidazolium tetrachloroferrate
• Horseradish peroxidase

Polyvinyl alcohol-ionic liquids (PVA-ILs) composite sol, graphenes( GS) and nano-Au were used to immobilize horseradish peroxidase(HRP). A novel hydrogen peroxide biosensor(HRP/PVA-ILs/HRP/nano Au-GS/GC) was obtained by immobilizing HRP with composite membrane of PVA-ILs on a glassy carbon (CC) electrode, which was modified with graphenes and nano Au. The experimental condition was optimized and the electrochemistry behavior of the HRP/PVA-ILs/HRP/nano Au-GS/GC biosensor in 0. 1 mol/L HAc-NaAc+0.1 mol/L KCl( pH =6. 5) including 11202 was discussed. The response of the sensor to 11202 is linear in the range of 9. 55x10(-6)-6.01x10(-3) mol/L with a correlation coefficient of 0.9990. The detection limit is 3.3 x10(-7) mol/L. The experiments show that the biosensor exhibits high sensitivity, good reproducibility and stability and longer service life.

• 出版日期2013-9-10
• 单位河南大学