Low dose radiation induced senescence of human mesenchymal stromal cells and impaired the autophagy process

作者：Alessio Nicola; Del Gaudio Stefania; Capasso Stefania; Di Bernardo Giovanni; Cappabianca Salvatore; Cipollaro Marilena; Peluso Gianfranco; Galderisi Umberto^*

来源：Oncotarget, 2015, 6(10): 8155-8166.

DOI：10.18632/oncotarget.2692

• Mesenchymal stem cells
• senescence
• radiation

Low doses of radiation may have profound effects on cellular function. Individuals may be exposed to low doses of radiation either intentionally for medical purposes or accidentally, such as those exposed to radiological terrorism or those who live near illegal radioactive waste dumpsites.
We studied the effects of low dose radiation on human bone marrow mesenchymal stromal cells (MSC), which contain a subpopulation of stem cells able to differentiate in bone, cartilage, and fat; support hematopoiesis; and contribute to body's homeostasis.
The main outcome of low radiation exposure, besides reduction of cell cycling, is the triggering of senescence, while the contribution to apoptosis is minimal. We also showed that low radiation affected the autophagic flux. We hypothesize that the autophagy prevented radiation deteriorative processes, and its decline contributed to senescence.
An increase in ATM staining one and six hours post-irradiation and return to basal level at 48 hours, along with persistent gamma-H2AX staining, indicated that MSC properly activated the DNA repair signaling, though some damages remained unrepaired, mainly in non-cycling cells. This suggested that the impaired DNA repair capacity of irradiated MSC seemed mainly related to the reduced activity of a non-homologous end-joining (NHEJ) system rather than HR (homologous recombination).

• 出版日期2015-4-10