Mapping biological composition through quantitative phase and absorption X-ray ptychography

作者：Jones Michael W M; Elgass Kirstin; Junker Mark D; Luu Mac B; Ryan Michael T; Peele Andrew G; van Riessen Grant A^*

来源：Scientific Reports, 2014, 4(1): 6796.

DOI：10.1038/srep06796

Isolating compositional information in biological X-ray imaging can be problematic as such information is conflated with thickness and density variations when viewing in projection through a sample. We demonstrate an effective method for identifying variations in material composition by simultaneously using the quantitative phase and magnitude images provided through soft X-ray ptychography. Using this approach we show significantly increased contrast and improved reliability of the identification of intracellular features from uncharacterised samples. While demonstrated for X-ray ptychography, this method is immediately applicable to electron and optical microscopy methods where the complex transmission function of the sample is recovered.

• 出版日期2014-10-28