Assessment of the reproducibility of oligoclonal IgM band detection for its application in daily clinical practice

作者：Espino M; Abraira V; Arroyo R; Bau L; Camara C; Campos Ruiz L; Casanova B; Espejo C; Fernandez O; Garcia Merino A; Garcia Sanchez M I; Gomez M; Gosis A; Izquierdo G; Meca J; Montalban X; Morandeira F; Olascoaga J; Prada A; Quintana E; Ramio Torrenta Ll; Rodriguez Antigueedad A; Salgado G; Santiago J L; Sarasola E; Simo Castello M; Alvarez Cermeno J C; Villar L M^*

来源：Clinica Chimica Acta, 2015, 438: 67-69.

DOI：10.1016/j.cca.2014.08.004

• Immunoglobulin IgM
• Oligoclonal bands
• Multiple sclerosis
• Biomarker

Background: The presence of oligoclonal IgM bands (OCMB) in cerebrospinal fluid (CSF) is an unfavourable prognostic marker in multiple sclerosis. There is no commercial test to investigate OCMB status. However, a sensitive and specific isoelectrofocusing (IEF) and western blot method was described. We aimed to study the inter-centre reproducibility of this technique, a necessary condition for a reliable test to be incorporated into clinical practice.
Methods: The presence of OCMB was analysed by IEF and western blot with prior reduction of pentameric IgM. We assayed the reproducibility of this test in a blinded multicentre study performed in 13 university hospitals. Paired-CSF and serum samples from 52 neurological patients were assayed at every centre.
Results: Global analysis rendered a concordance of 89.8% with a kappa value of 0.71.
Conclusion: These data indicate that OCMB detection by means of IEF and western blot with IgM reduction shows a good interlaboratory reproducibility and thus can be used in daily clinical setting. 2014 Elsevier B.V.

• 出版日期2015-1-1