科研之友
微信
新浪微博
Facebook
分享链接
摘要
An acetonitrile-salt stacking method was established for the assay of lipoic acid (LA) in biological samples. Samples were deproteinized with acetonitrile at a final concentration of 60 % (v/v) and then injected hydrodynamically at 3.45 x 10(3) Pa for 180.0 s. The optimum background electrolyte was found to be 90.0 mmol L-1 pH 9.1 borate buffer. LA could be detected within 35 min at +7.0 kV with satisfactory repeatability (relative standard deviations, RSDs, of migration times and peak areas were both below 10 % for intraday and interday; n = 6/9) and a relatively low limit of detection of ca. 0.5 mu mol L-1.
- 出版日期2014-1
- 单位西安交通大学
