An acetylcholinesterase-independent mechanism for neurobehavioral impairments after chronic low level exposure to dichlorvos in rats (Retracted article. See vol. 96, pg. 114, 2010)

作者：Verma Suresh Kumar; Kumar Vijay; Gill Kiran Dip^*

来源：Pharmacology Biochemistry and Behavior, 2009, 92(1): 173-181.

DOI：10.1016/j.pbb.2008.11.010

• Organophosphate
• Dichlorvos
• Neurotoxicity
• Muscarinic receptors
• Neurobehavior
• Morris Water Maze

The present study was designed to explore an alternate mechanism of action other than acetylcholinesterase inhibition for the chronic, low-level exposure to dichlorvos, an organophosphate, in vivo. Dichlorvos, at dose of 1.0 as well as 6.0 mg/kg b. wt. for 12 weeks to rats showed impairment in neurobehavioral indices viz. rota rod, passive avoidance and water maze tests. Though higher dose of dichlorvos had a detrimental effect on acetylcholinesterase activity. no significant inhibition was seen with lower dose of dichlorvos for the same period of exposure i.e. 12 weeks. Muscarinic acetylcholine receptor binding studies revealed a decrease in the number of binding sites (B(max)) in low as well as high dose groups but the dissociation constant (K(d)) value was unaffected with both doses of dichlorvos. Use of selective ligands against M(1), M(2) and M(3) receptor subtypes indicated that M(2)dd is the major receptor subtype being affected by chronic low-level exposure to dichlorvos. Western blot analysis and immunofluorescence studies also confirmed these biochemical findings. Thus, the present study suggests that M2 receptors may play a major role in the development of neurobehavioral impairments after chronic exposure to dichlorvos.

• 出版日期2009-3
• 单位河北医科大学