mu-Calpain mediates hippocampal neuron death in rats after lithium-pilocarpine-induced status epilepticus

作者：Wang Shuhua; Wang Shengjun; Shan Peiyan; Song Zhaofeng; Dai Tingjun; Wang Rong; Chi Zhaofu^*

来源：Brain Research Bulletin, 2008, 76(1-2): 90-96.

DOI：10.1016/j.brainresbull.2007.12.006

• mu-calpain
• programmed cell death
• status epilepticus
• caspase-3
• pilocarpine
• hippocampus

Status epilepticus (SE) is a severe clinical manifestation of epilepsy which causes brain damage. The pathological process and underlying mechanisms involved in the programmed cell death (PCD) are still not fully clear. In the current study, rats were induced SE by lithium-pilocarpine administration. Our data showed hippocampal neurons death appeared at 6 h after SE and sustained for 7 days. By blotting the activation of mu-calpain and its specific cleavage of nonerythroid alpha-spectrin (alpha SpII) (145 kDa) was evident at 1 and 3 days after SE, which coincided with Bid activation, apoptosis inducing factor (AIF) translocation and cytochrome c release from mitochondria, whereas, activated caspase-3 and caspase-3-specific fragments of alpha SpII (120 kDa) predominantly appeared at 5 and 7 days after SE. Moreover, MDL-28170, a calpain inhibitor, partially rescued the neuron death and attenuated the expression of activated mu-calpain, cleavage of Bid (15 kDa), AIF translocation and cytochrome c release. Taken together, our study indicated that mu-calpain mediated hippocampal neuron PCD is prior to caspase-3 activation. It functioned via translocation of Bid, AIF and cytochrome c release.

• 出版日期2008-5-15
• 单位山东大学