Untargeted metabolomics analysis reveals dynamic changes in azelaic acid- and salicylic acid derivatives in LPS-treated Nicotiana tabacum cells

作者：Mhlongo M I; Tugizimana F; Piater L A; Steenkamp P A; Madala N E; Dubery I A^*

来源：Biochemical and Biophysical Research Communications, 2017, 482(4): 1498-1503.

DOI：10.1016/j.bbrc.2016.12.063

• Azelaic acid
• Innate immunity
• Lipopolysaccharide
• Metabolomics
• Phytohormones
• Salicylic acid

To counteract biotic stress factors, plants employ multilayered defense mechanisms responsive to pathogen-derived elicitor molecules, and regulated by different phytohormones and signaling molecules. Here, lipopolysaccharide (LPS), a microbe-associated molecular pattern (MAMP) molecule, was used to induce defense responses in Nicotiana tabacum cell suspensions. Intracellular metabolites were extracted with methanol and analyzed using a liquid chromatography-mass spectrometry (UHPLC-qTOF-MS/MS) platform. The generated data were processed and examined with multivariate and univariate statistical tools. The results show time-dependent dynamic changes and accumulation of glycosylated signaling molecules, specifically those of azelaic acid, salicylic acid and methyl-salicylate as contributors to the altered metabolomic state in LPS-treated cells.

• 出版日期2017-1-22