Comparison of two methods for measurement of equine insulin

作者：Banse Heidi E^*; McCann Joseph; Yang Fan; Wagg Catherine; McFarlane Dianne

来源：Journal of Veterinary Diagnostic Investigation, 2014, 26(4): 527-530.

DOI：10.1177/1040638714536560

• Bias
• chemiluminescent immunoassay
• insulin
• linearity
• radioimmunoassay
• recovery

Diagnosis of equine hyperinsulinemia requires an accurate method for quantification of equine insulin concentrations. The objectives of the current study were to compare 2 commercially available techniques for measurement of equine insulin, the radioimmunoassay (RIA) and chemiluminescent immunoassay (CIA). Recovery was poor for both assays, but worse for the CIA. Serial dilution of a high endogenous insulin sample yielded better linearity for the RIA (r(2) = 0.99, P %26lt; 0.001) than the CIA (r(2) = 0.92, P = 0.009). Bland-Altman analysis indicated that the CIA was, on average, 91 pmol/l higher than the RIA, with wide limits of agreement (95% limits of agreement: -508 to 691 pmol/l). These findings suggest that results between the assays should not be considered interchangeable.

• 出版日期2014-7