An alternative method to recover Toxoplasma gondii from greenery and fruits

作者：de Souza Carla Zangari^*; Rafael Katyelle; Sanders Ana Paula; Tiyo Bruna Tiaki; Marchioro Ariella Andrade; Colli Cristiane Maria; Gomes Monica Lucia; Falavigna Guilherme Ana Lucia

来源：International Journal of Environmental Health Research, 2016, 26(5-6): 600-605.

DOI：10.1080/09603123.2016.1227960

• Toxoplasma gondii
• methods
• membrane filtration
• PCR

Toxoplasma gondii oocysts are an important form of contamination with a high dispersion in the environment, but their detection is still a challenge. This study evaluated the recovery of oocysts from strawberries and crisphead lettuce. Samples (250g of strawberries or one head of lettuce) were experimentally inoculated with 10, 10(2), 10(3) and 10(4)T. gondii oocysts, by two separate processes, spot dripping and immersion. Then, 50g of each sample was washed, filtered through a cellulose ester membrane, and concentrated by centrifugation. Three aliquots were taken for DNA extraction in a direct way, after freeze-thaw (FT) cycles or ultrasound (US), followed by PCR (B22-B23 and Tox4-Tox5 primers). The T. gondii DNA was amplified with the primers B22-B23 in all samples contaminated by dripping and when DNA extraction was carried out after FT or US. These techniques may be useful in epidemiological surveillance in the control of this zoonosis.

• 出版日期2016-12