Analysis of PRODUCTION OF FLAVONOL GLYCOSIDES-dependent flavonol glycoside accumulation in Arabidopsis thaliana plants reveals MYB11-, MYB12-and MYB111-independent flavonol glycoside accumulation

作者：Stracke Ralf^*; Jahns Oliver; Keck Matthias; Tohge Takayuki; Niehaus Karsten; Fernie Alisdair R; Weisshaar Bernd

来源：NEW PHYTOLOGIST, 2010, 188(4): 985-1000.

DOI：10.1111/j.1469-8137.2010.03421.x

• Arabidopsis thaliana
• flavonoid biosynthesis
• flavonol glycosides
• flavonols
• gene regulation
• R2R3-MYB

P>The flavonol branch of flavonoid biosynthesis is under transcriptional control of the R2R3-MYBs PRODUCTION OF FLAVONOL GLYCOSIDE1 (PFG1/MYB12, PFG2/MYB11 and PFG3/MYB111) in Arabidopsis thaliana. Here, we investigated the influence of specific PFG transcription factors on flavonol distribution in various organs.
A combination of genetic and metabolite analysis was used to identify transcription factor gene-metabolite correlations of the flavonol metabolic pathway. Flavonol glycoside accumulation patterns have been analysed in wild-type and multiple R2R3-MYB PFG mutants in an organ- and development-dependent manner using high-performance thin-layer chromatography, supplemented with liquid chromatography-mass spectroscopy metabolite profiling.
Our results clearly demonstrate a differential influence of MYB11, MYB12 and MYB111 on the spatial accumulation of specific flavonol derivatives in leaves, stems, inflorescences, siliques and roots. In addition, MYB11-, MYB12- and MYB111-independent flavonol glycoside accumulation was observed in pollen grains and siliques/seeds.
The highly complex tissue- and developmental-specific regulation of flavonol biosynthesis in A. thaliana is orchestrated by at least four PFG transcription factors, differentially influencing the spatial accumulation of specific flavonol derivatives. We present evidence that a separate flavonol control mechanism might be at play in pollen.

• 出版日期2010