ACOUSTICAL PROPERTIES OF INDIVIDUAL LIPOSOME-LOADED MICROBUBBLES

作者：Luan Ying^*; Faez Telli; Gelderblom Erik; Skachkov Ilya; Geers Bart; Lentacker Ine; van der Steen Ton; Versluis Michel; de Jong Nico

来源：Ultrasound in Medicine and Biology, 2012, 38(12): 2174-2185.

DOI：10.1016/j.ultrasmedbio.2012.07.023

• Liposome-loaded microbubbles
• Shell characterization
• Ultrasound contrast agents
• Drug delivery

A comparison between phospholipid-coated microbubbles with and without liposomes attached to the microbubble surface was performed using the ultra-high-speed imaging camera (Brandaris 128). We investigated 73 liposome-loaded microbubbles (loaded microbubbles) and 41 microbubbles without liposome loading (unloaded microbubbles) with a diameter ranging from 3-10 mu m at frequencies ranging from 0.6-3.8 MHz and acoustic pressures ranging from 5-100 kPa. The experimental data showed nearly the same shell elasticity for the loaded and unloaded bubbles, but the shell viscosity was higher for loaded bubbles compared with unloaded bubbles. For loaded bubbles, a higher pressure threshold for the bubble vibrations was noticed. In addition, an %26quot;expansion-only%26apos;%26apos; behavior was observed for up to 69% of the investigated loaded bubbles, which mostly occurred at low acoustic pressures (%26lt;= 30 kPa). Finally, fluorescence imaging showed heterogeneity of liposome distributions of the loaded bubbles. (E-mail: y.luan@erasmusmc.

• 出版日期2012-12