A Window Into Clinical Next-Generation Sequencing-Based Oncology Testing Practices

作者：Nagarajan Rakesh^*; Bartley Angela N; Bridge Julia A; Jennings Lawrence J; Kamel Reid Suzanne; Kim Annette; Lazar Alexander J; Lindeman Neal I; Moncur Joel; Rai Alex J; Routbort Mark J; Vasalos Patricia; Merker Jason D

来源：Archives of Pathology & Laboratory Medicine, 2017, 141(12): 1679-1685.

DOI：10.5858/arpa.2016-0542-CP

Context.-Detection of acquired variants in cancer is a paradigm of precision medicine, yet little has been reported about clinical laboratory practices across a broad range of laboratories.
Objective.-To use College of American Pathologists proficiency testing survey results to report on the results from surveys on next-generation sequencing-based oncology testing practices.
Design.-College of American Pathologists proficiency testing survey results from more than 250 laboratories currently performing molecular oncology testing were used to determine laboratory trends in next-generation sequencing-based oncology testing.
Results.-These presented data provide key information about the number of laboratories that currently offer or are planning to offer next-generation sequencing-based oncology testing. Furthermore, we present data from 60 laboratories performing next-generation sequencing-based oncology testing regarding specimen requirements and assay characteristics. The findings indicate that most laboratories are performing tumor-only targeted sequencing to detect single-nucleotide variants and small insertions and deletions, using desktop sequencers and predesigned commercial kits. Despite these trends, a diversity of approaches to testing exists.
Conclusions.-This information should be useful to further inform a variety of topics, including national discussions involving clinical laboratory quality systems, regulation and oversight of next-generation sequencing-based oncology testing, and precision oncology efforts in a data-driven manner.

• 出版日期2017-12
• 单位西北大学