Aspects of the Synthesis of an Exceptionally Preorganized Self-Immolative Spacer-Phenolate Unit

作者：Waibel Michael; Zhang Xiao Bing; Hasserodt Jens^*

来源：Synthesis, 2009, (2): 318-324.

DOI：10.1055/s-0028-1083296

• self-immolative spacer
• preorganization
• tertiary ether formation
• low temperature hydrogenation
• fluorescence

This study introduces a highly preorganized self-immolative spacer that is coupled to a nonfluorescent leaving group. The water-soluble compound can rapidly liberate a water-insoluble fluorescent precipitate by intramolecular attack of a free amine on a phenolic ester group via a six-membered transition state. One of the crucial steps in the synthesis of this molecule was a sterically very unfavored nucleophilic substitution to create a tertiary ether; this was optimized using model compounds. Another key challenge was deprotection by catalytic hydrogenation at low temperatures in order to furnish the hydroacetate of the free amine without further fragmentation. LC-MS and fluorescence studies showed that this compound collapsed instantaneously in methanol, as well as within a wide pH range in buffered aqueous media.

• 出版日期2009-1-16