New Technique for Preparing Cartilage for Intracordal Injection: The Freezing and Grinding Method

作者：Park Young Min; Lee Won Yong; Lim Yun Sung; Lee Jin Choon; Lee Byung Joo^*; Wang Soo Geun

来源：Journal of Voice, 2014, 28(4): 508-511.

DOI：10.1016/j.jvoice.2014.01.001

• Injection laryngoplasty
• Intracordal cartilage injection

Objectives/Hypothesis. We developed a technique for preparing harvested cartilage that creates finer, more uniform pieces by freezing with liquid nitrogen and grinding with a mortar and pestle. Herein, we report the application of this new technique for intracordal cartilage injection. %26lt;br%26gt;Study Design. Experimental study. %26lt;br%26gt;Methods. Human cartilage was obtained from surgical cases. In the standard method, harvested cartilage was prepared with scissors and a knife. In the experimental group, harvested cartilage was frozen with liquid nitrogen and ground with a mortar and pestle. %26lt;br%26gt;Results. It took an average of 60 minutes to manipulate cartilage using the standard technique, whereas it took an average of 10 minutes using the freezing and grinding method (P %26lt; 0.001). The average size of cartilage flakes generated by the standard and new techniques were 727 and 48.6 mu m, respectively. The cartilage flakes produced using scissors and a knife were able to pass through a 19-gauge needle, whereas those created using the freezing and grinding method were able to pass through a 24-gauge needle. %26lt;br%26gt;Conclusions. Using the freezing and grinding method, cartilage was broken into fine, uniform pieces that could pass through a 24-gauge needle. This new technique will facilitate the production of commercial cartilage material for intracordal injection.

• 出版日期2014-7