APC/C-Cdh1 Enables Removal of Shugoshin-2 from the Arms of Bivalent Chromosomes by Moderating Cyclin-Dependent Kinase Activity

作者：Rattani Ahmed; Mejia Randy Ballesteros; Roberts Katherine; Roig Maurici B; Godwin Jonathan; Hopkins Michael; Eguren Manuel; Sanchez Pulido Luis; Okaz Elwy; Ogushi Sugako; Wolna Magda; Metson Jean; Pendas Alberto M; Malumbres Marcos; Novak Bela; Herbert Mary; Nasmyth Kim^*

来源：Current Biology, 2017, 27(10): 1462-+.

DOI：10.1016/j.cub.2017.04.023

In mammalian females, germ cells remain arrested as primordial follicles. Resumption of meiosis is heralded by germinal vesicle breakdown, condensation of chromosomes, and their eventual alignment on metaphase plates. At the first meiotic division, anaphase-promoting complex/cyclosome associated with Cdc20 (APC/C-Cdc20) activates separase and thereby destroys cohesion along chromosome arms. Because cohesion around centromeres is protected by shugoshin-2, sister chromatids remain attached through centromeric/pericentromeric cohesin. We show here that, by promoting proteolysis of cyclins and Cdc25B at the germinal vesicle (GV) stage, APC/C associated with the Cdh1 protein (APC/C-Cdh1) delays the increase in Cdk1 activity, leading to germinal vesicle breakdown (GVBD). More surprisingly, by moderating the rate at which Cdk1 is activated following GVBD, APC/C-Cdh1 creates conditions necessary for the removal of shugoshin-2 from chromosome arms by the Aurora B/C kinase, an event crucial for the efficient resolution of chiasmata.

• 出版日期2017-5-22
• 单位河北医科大学