Blood Vessel Density in De Novo Formed Adipose Tissue Is Decreased Upon Overexpression of TIMP-1

作者：Scroyen Ilse; Jacobs Frank; Cosemans Leentje; De Geest Bart; Lijnen Henri R^*

来源：Obesity, 2010, 18(3): 638-640.

DOI：10.1038/oby.2009.279

Matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) play a role in the development of obesity by contributing to adipogenesis, angiogenesis, and extracellular matrix degradation. We have evaluated a potential functional role of TIMP-1, which inhibits most MMPs, in in vivo adipogenesis. Therefore, human (h) TIMP-1 was overexpressed by injection in the tail vein of NUDE mice of an adenoviral vector 3 days before injection of 3T3-F442A preadipocytes in the back. After 4 weeks of high-fat diet, the de novo formed fat was analyzed. Overexpression of hTIMP-1 had no effect on de novo formed fat pad mass. However, the blood vessel density of the fat pads from mice overexpressing hTIMP-1 was significantly lower than in controls (587 /- 11 mm(-2) vs. 806 /- 20 mm(-2), P < 0.0001) whereas the adipocytes were somewhat larger (1,477 /- 44 mu m(2) vs. 1,285 /- 32 mu m(2), P = 0.03). Thus, in vivo hTIMP-1 overexpression did not significantly affect the extent of de novo adipose tissue formation, but was associated with significantly lower blood vessel density.

• 出版日期2010-3