Alpha-2, 3-sialyltransferases regulate the multidrug resistance of chronic myeloid leukemia through miR-4701-5p targeting ST3GAL1

作者：Li, Yan; Luo, Shihua; Dong, Weijie; Song, Xiaobo; Zhou, Huimin; Zhao, Lifen; Jia, Li^*

来源：Laboratory Investigation, 2016, 96(7): 731-740.

DOI：10.1038/labinvest.2016.50

• BREAST-CANCER
• MICRORNAS
• SIALYLTRANSFERASE
• CELLS
• EXPRESSION
• TRANSCRIPTION

The aberrant sialylation profile on the surface of leukemia cells has been recognized for its potential diagnostic value towards assessing leukemia multidrug resistance (MDR). MicroRNAs as endogenous regulators of gene expression have been implicated in treating MDR. In this study, we describe the differential expressional profiles of alpha-2, 3-sialyltransferases (ST) and miR-4701-5p in three pairs of chronic myeloid leukemia (CML) cell lines and 48 clinical samples of bone marrow mononuclear cells from CML patients. The altered expression level of ST3GAL1 was found corresponding to the drug resistant phenotype (with and without adriamycin resistance) of CML cell lines both in vitro and in vivo. Further the results showed that miR-4701-5p directly targeted ST3GAL1 to reduce CML cells resistance to multiple chemotherapeutics in vitro and to convert tumor cells from adriamycin resistant to susceptible in vivo of mice. These results indicate that differential expression of alpha-2,3 ST is involved in MDR of CML, and that miR-4701-5p regulates the susceptibility of CML cells to multiple drugs, at least in part, through targeting ST3GAL1.

• 出版日期2016-7
• 单位上海市闵行区中心医院; 大连医科大学