Antinociceptive esters of N-methylanthranilic acid: Mechanism of action in heat-mediated pain

作者：Gomes Pinheiro Mariana Martins; Radulovic Niko S; Miltojevic Ana B; Boylan Fabio; Fernandes Patricia Dias^*

来源：European Journal of Pharmacology, 2014, 727: 106-114.

DOI：10.1016/j.ejphar.2013.12.042

• Ternanthranin
• N-methylanthranilates
• Antinociceptive activity
• Pain
• Analgesia

Recently, we identified a new natural antinociceptive alkaloid ternanthranin, isopropyl N-methylanthranilate (ISOAN), from the plant species Choisya ternata Kunth (Rutaceae). In this work we concentrated on the elucidation of its mechanism of action in comparison with two other esters of this acid (methyl (MAN) and propyl (PAN)). Mice orally pre-treated with ISOAN, MAN or PAN (at 0.3, 1 and 3 mg/kg) were less sensitive to chemical or thermal stimuli in different nociception models (formalin-, capsaicin- and glutamate induced licking response, tail flick and hot plate). All compounds (1 and 3 mg/kg) showed significant activity in the peripheral nociception models, as well as a dose dependent spinal antinociceptive effect in the tail flick model. We observed that glibenclamide was able to reverse the antinociceptive effect of ISOAN in the hot plate model suggesting the involvement of K-ATP(+) channels. The antinociceptive effect of MAN and PAN may be related to adrenergic, niirergic and seroloninergic pathways. In addition, the antinociception of PAN was reverted by naloxone implying that the opioid pathway participates in its activity. The cholinergic and cannabinoid systems were found not be involved in the onset of the antinociceptive effects of any of the esters. In conclusion, isopropyl, methyl and propyl N-methylanthranilates produced significant peripheral and central antinociception at doses lower than that of morphine, the classical opioid analgesic drug, without causing toxicity.

• 出版日期2014-3-15