ARF6-mediated endosomal transport of Telencephalin affects dendritic filopodia-to-spine maturation

作者：Raemaekers Tim^*; Peric Aleksandar; Baatsen Pieter; Sannerud Ragna; Declerck Ilse; Baert Veerle; Michiels Christine; Annaert Wim

来源：The EMBO Journal, 2012, 31(15): 3252-3269.

DOI：10.1038/emboj.2012.182

• ARF6
• EFA6A
• flotillin
• spine morphogenesis
• Telencephalin

Dendritic filopodia are dynamic structures thought to be the precursors of spines during synapse development. Morphological maturation to spines is associated with the stabilization and strengthening of synapses, and can be altered in various neurological disorders. Telencephalin (TLN/intercellular adhesion molecule-5 (ICAM5)) localizes to dendritic filopodia, where it facilitates their formation/maintenance, thereby slowing spine morphogenesis. As spines are largely devoid of TLN, its exclusion from the filopodia surface appears to be required in this maturation process. Using HeLa cells and primary hippocampal neurons, we demonstrate that surface removal of TLN involves internalization events mediated by the small GTPase ADP-ribosylation factor 6 (ARF6), and its activator EFA6A. This endocytosis of TLN affects filopodia-to-spine transition, and requires Rac1-mediated dephosphorylation/release of actin-binding ERM proteins from TLN. At the somato-dendritic surface, TLN and EFA6A are confined to distinct, flotillin-positive membrane subdomains. The co-distribution of TLN with this lipid raft marker also persists during its endosomal targeting to CD63-positive late endosomes. This suggests a specific microenvironment facilitating ARF6-mediated mobilization of TLN that contributes to promotion of dendritic spine development. The EMBO Journal (2012) 31, 3252-3269. doi:10.1038/emboj.2012.182; Published online 10 July 2012

• 出版日期2012-8-1