Macromolecular properties and hypolipidemic effects of four sulfated polysaccharides from sea cucumbers

作者：Li, Shan; Li, Junhui; Zhi, Zijian; Wei, Chaoyang; Wang, Wenjun; Ding, Tian; Ye, Xingqian; Hu, Yaqin; Linhardt, Robert J.; Chen, Shiguo^*

来源：Carbohydrate Polymers, 2017, 173: 330-337.

DOI：10.1016/j.carbpol.2017.05.063

• Sulfated polysaccharides from sea cucumbers
• Chain conformation
• Anti-hyperlipidemic activity
• Structure -function relationship

The present study investigates the relationship between the high-order structure and hypolipidemic activity of four well-defined sulfated fucans from sea cucumber. The chain conformation, determined by a combination of AFM and SEC-MALLS-RI, indicate that fucosylated chondroitin sulfate (fCS) from Pearsono thuria graeffei (fCS-Pg) and Isostichopus badionotus (fCS-Ib), and fucoidan from Pgraeffei (fuc-Pg) were assigned as a random coil conformation with polysaccharide chain outstretched, while I. badionotus (fuc-Ib) was assigned as a spherical conformation and exhibited high viscosity. Fuc-Pg and fuc-Ib with higher molecular weights had a greater impact in inhibiting pancreatic lipase activity in vitro. However, fCS-Pg, fCS-lb and fuc-Pg with random linear conformation exhibited excellent hypolipidemic activity in SpragueDawley rats (SD rats) fed on high-fat diet (HFD), whereas fuc-Ib showed only a modest effect. Our results indicate that structural characteristics, including side branch and sulfation pattern can affect the chain conformation of polysaccharides, which determine their physicochemical properties and hypolipidemic activity.

• 出版日期2017-10-1
• 单位浙江大学