Diffusion-controlled Detection of Glucose with Microelectrodes in Mediated Bioelectrocatalytic Oxidation

作者:Noda Tatsuo; Wanibuchi Mizue; Kitazumi Yuki; Tsujimura Seiya; Shirai Osamu; Yamamoto Masahiro; Kano Kenji*
来源:Analytical Sciences, 2013, 29(3): 279-281.
DOI:10.2116/analsci.29.279

摘要

This paper describes a diffusion-controlled electrolysis of glucose with mediated bioelectrocatalysis at microdisk-electrodes. Under conditions of an extremely fast enzyme reaction, compared with the diffusion of glucose, the current in potential-step chronoamperometry reaches an almost steady state within 10 s, and is proportional to the glucose concentration. The current can be detected at +0.1 V (vs. AglAgCl) with 9,10-phenanthrenequinone as a mediator. The addition of carboxymethylcellulose increased the linear range up to 10 mM.

  • 出版日期2013-3