A novel biosensor based on microbial electrolysis cells for rapid determination of biochemical oxygen demand

作者：Jiang Hai Ming^*; Si Wan Tong; Pan Jian Gang

来源：Journal of Chemical Engineering of Chinese Universities, 2015, 29(6): 1489-1495.

DOI：10.3969/j.issn.1003-9015.2015.06.029

• Artificial wastewater
• Biochemical oxygen demands (BOD)
• Electrochemically-active microbe
• High sensitivity
• Linear relationships
• Measurement time
• Relative standard deviations
• Wide-linear range

A novel microbial electrolysis cell (MEC) based biosensor for rapid determination of biochemical oxygen demand (BOD) was developed, and its performance was evaluated with glucose-glutamic acid containing artificial wastewater. The results show that when the applied voltage is kept at 0.7 V, the maximum current of the biosensor follows Monod equation under BOD concentration of 10~400 mg×L-1, and the maximum current has linear relationship with BOD when the BOD concentration is in the range of 10~100 mg×L-1. The results also indicate that the measurement time is about 10 min when the BOD concentration is in the range of 10~400 mg×L-1. The relative standard deviation of repeatability was less than ±12.2%, while the relative standard deviation of stability was less than ±6% over a period of 12 days. These results demonstrate that the development of novel biosensors based on MEC for rapid determination of BOD is feasible, and the biosensor has advantages of high sensitivity, wide linear range, short detection time, good repeatability and good stability.

• 出版日期2015
• 单位内蒙古大学