A sequence-based typing method for HLA-DPB1 genotyping

作者：Liu Z; Lin J; Fu Z; Pan D; Xu A

来源：Acta Scientiarum Naturalium Universitatis Sunyatseni, 2001, 40(5): 83-87.

An automatic sequence-based typing (SBT) method is established for genotyping. It is a high-resolution genotyping method by utilizing the automatic genotyping program (AGP) to compare the DNA sequence data obtained by direct sequencing of PCR products with a database containing known allelic sequences. It offers SBT the ability to distinguish heterozygous individuals as well as identifying homozygous individuals. To validate this typing system, 50 unrelated southern Chinese individuals were typed in the HLA-DPB1 locus as an example. Two ambiguous heterozygotes and one novel allelic sequence were found in these individuals. The typing results were confirmed by cloning and subsequent sequencing of the cloned DNA without any discordance except two ambiguous heterozygotes, indicated a 96% concordance. Thus, an efficient and accurate SBT method was established by using AGP program. It was proved applicable in genotyping of HLA-DPB1 locus, and can be easily applied and extended to other gene.

• 出版日期2001