A novel strategy to improve the aromatic alcohols tolerance of enzyme for preparative-scale synthesis of natural glycosides

作者：Zhou Youzhi; Zhao Lu; Liu Ke; Zhang Jinsong; Chu Jianlin; He Bingfang

来源：Catalysis Communications, 2017, 102: 9-12.

DOI：10.1016/j.catcom.2017.07.025

• Enzyme inactivation
• Substrate tolerance
• Hydrophilic solvent
• Preparative-scale
• Natural glycoside

Aromatic alcohols are liable to result in enzyme inactivation. A novel strategy was established for improving the aromatic alcohol tolerance of β-galactosidase (BMG) from Bacillus megaterium YZ08. The half-life of BMG in 200 mM vanillyl alcohol solution was dramatically increased by 9–123 times with the addition of hydrophilic solvents. In 30% DMSO, the reaction concentration of aromatic alcohol could reach up to 300–400 mM, and 0.85–2.44 g of natural glycosides were successfully obtained in 100 mL-scale. The simple and effective strategy shows potential applications when dealing with the preparative-scale exploitation of enzymatic reactions.

• 出版日期2017-12