Autophagy Protects MC3T3-E1 Cells upon Aluminum-Induced Apoptosis

作者：Yang, Xu; Zhang, Jian; Ji, Qiang; Wang, Fan; Song, Miao; Li, Yanfei^*

来源：Biological Trace Element Research, 2018, 185(2): 433-439.

DOI：10.1007/s12011-018-1264-7

• Aluminum
• MC3T3-E1 cell
• Autophagy
• Apoptosis

Aluminum (Al) exposure has adverse effects on osteoblasts, and the effect might be through autophagy-associated apoptosis. In this study, we showed that aluminum trichloride (AlCl3) could induce autophagy in MC3T3-E1 cells, as demonstrated by monodansylcadaverine (MDC) staining and the expressions of the ATG3, ATG5, and ATG9 genes. We found AlCl3 inhibited MC3T3-E1 cell survival rate and caused apoptosis, as evidenced by CCK-8 assay, Annexin V/PI double staining, and increased expressions of Bcl-2, Bax, and Caspase-3 genes. In addition, increased autophagy induced by rapamycin further attenuated the MC3T3-E1 cell apoptosis rate after AlCl3 exposure. These results support the hypothesis that autophagy plays a protective role in impeding apoptosis caused by AlCl3. Activating autophagy may be a strategy for treatment of Al-induced bone disease.

• 单位
  东北农业大学