Substrate Specificity of the Magnolia Flower Polyphenol Oxidase Separated on the Cation Exchanger and Hydrophobic Interaction Column

作者：Kim Jae Joon; Kim Woo Yeon^*

来源：Journal of the Korean Society for Applied Biological Chemistry, 2013, 56(6): 755-757.

DOI：10.1007/s13765-013-3223-5

• Cation exchanger
• Magnolia flower polyphenol oxidase
• Substrate specificity

Polyphenol oxidase (PPO) was separated from Magnolia (Magnolia kobus) flower by acetone precipitation and CM-Sepharose and Phenyl-Sepharose chromatographies. Molecular weight of the purified PPO from Magnolia flower was assumed to be just over 20 kDa on the sodiumdodecylsulfate-polyacrylamide gel electrophoresis and around 40 kDa under non-boiling without beta-mercaptoethanol. Magnolia flower PPO showed the highest enzyme activity with chlorogenic acid as a substrate.

• 出版日期2013-12