Nondestructive micropatterning of living animal cells using focused femtosecond laser-induced impulsive force

作者：Kaji Takahiro^*; Ito Syoji; Miyasaka Hiroshi; Hosokawa Yoichiroh; Masuhara Hiroshi; Shukunami Chisa; Hiraki Yuji

来源：Applied Physics Letters, 2007, 91(2): 023904.

DOI：10.1063/1.2753103

Micropatterning of mouse NIH3T3 fibroblast cells was performed using focused femtosecond laser-induced impulsive force in a culture medium. The cells were detached from an upper substrate by the force and transferred to an underlying substrate with less than spatial resolution of 80 mu m full width at half maximum. About 80% of the cells were confirmed to be alive at 3 h after the patterning. The force exerted to the cell was investigated by high-speed imaging and estimated to be an order of micronewtons. The force origin was not only due to cavitation bubble but also due to shockwave and jet flow.

• 出版日期2007-7-9