Aspirin-triggered resolvin D1 reduces mucosal inflammation and promotes resolution in a murine model of acute lung injury

作者：Eickmeier O; Seki H; Haworth O; Hilberath J N; Gao F; Uddin M; Croze R H; Carlo T; Pfeffer M A; Levy B D^*

来源：Mucosal Immunology, 2013, 6(2): 256-266.

DOI：10.1038/mi.2012.66

Acute lung injury (ALI) is a severe illness with excess mortality and no specific therapy. Protective actions were recently uncovered for docosahexaenoic acid-derived mediators, including D-series resolvins. Here, we used a murine self-limited model of hydrochloric acid-induced ALI to determine the effects of aspirin-triggered resolvin D1 (AT-RvD1; 7 S,8 R,17 R -trihydroxy-4 Z,9 E,11 E,13 Z,15 E,19 Z -docosahexaenoic acid) on mucosal injury. RvD1 and its receptor ALX/FPR2 were identified in murine lung after ALI. AT-RvD1 ( 0.5-5 mu gkg(-1)) decreased peak inflammation, including bronchoalveolar lavage fluid (BALF) neutrophils by 75 %. Animals treated with AT-RvD1 had improved epithelial and endothelial barrier integrity and decreased airway resistance concomitant with increased BALF epinephrine levels. AT-RvD1 inhibited neutrophil-platelet heterotypic interactions by downregulating both P-selectin and its ligand CD24. AT-RvD1 also significantly decreased levels of BALF pro-inflammatory cytokines, including interleukin (IL)-1 beta, IL-6, Kupffer cells, and tumor necrosis factor- , and decreased nuclear factor- B-phosphorylated p65 nuclear translocation. Taken together, these findings indicate that AT-RvD1 displays potent mucosal protection and promotes catabasis after ALI.

• 出版日期2013-3