The Role of S100B in the Interaction Between Adipocytes and Macrophages

作者：Fujiya Atsushi; Nagasaki Hiroshi; Seino Yusuke; Okawa Tetsuji; Kato Jiro; Fukami Ayako; Himeno Tatsuhito; Uenishi Eita; Tsunekawa Shin; Kamiya Hideki; Nakamura Jiro; Oiso Yutaka; Hamada Yoji^*

来源：Obesity, 2014, 22(2): 371-379.

DOI：10.1002/oby.20532

Objective: The S100 calcium binding protein B (S100B) implicated in brain inflammation acts via the receptor of advanced glycation end products (RAGE) and is also secreted from adipocytes. We investigated the role of S100B in the interaction between adipocytes and macrophages using a cell-culture model.
Design and Methods: RAW264.7 macrophages (RAW) were stimulated by recombinant S100B to observe alterations in TNF-alpha and M1 markers; 3T3-L1 adipocytes (L1) were stimulated by TNF-alpha to examine S100B secretion. RAW and L1 were then mutually stimulated with conditioned media of each other, or co-cultured. The effects of S100B silencing or a RAGE-neutralizing antibody were also investigated.
Results: S100B upregulated TNF-alpha and M1 markers in RAW, and TNF-alpha augmented S100B secretion from L1. L1 conditioned media stimulated TNF-alpha secretion from RAW, and RAW conditioned media increased S100B secretion from L1. The co-culture of RAW and L1 increased TNF-alpha, S100B, and the expression of M1 markers and the MCP-1 receptor CCR2. The silencing of S100B or RAGE neutralization significantly ameliorated TNF-alpha hypersecretion from RAW that were stimulated with L1 conditioned media.
Conclusions: Thus, S100B as an adipokine may play a role in the interaction between adipocytes and macrophages to establish a vicious paracrine loop.

• 出版日期2014-2