Use of gamma-aminopropyl-coated glass surface for the patterning of oligonucleotides through oxime bond formation

作者：Dendane Nabil; Hoang Antoine; Defrancq Eric^*; Vinet Francoise; Dumy Pascal

来源：Bioorganic & Medicinal Chemistry Letters, 2008, 18(8): 2540-2543.

DOI：10.1016/j.bmcl.2008.03.054

• DNA
• lab on a chip
• micro-array
• oxime
• photo-deprotection
• surface patterning

The present work reports on the preparation of glass surfaces coated with NPPOC-protected aminooxy groups and their use for the patterning of oligonucleotides on glass slides and in capillary tubes. The method involves the use of surfaces coated with amino groups using (gamma-aminopropyl)triethoxy silane and subsequent grafting of the aminooxy groups by using the activated ester 1. The NPPOC-protected aminooxy groups on the surfaces can be cleaved upon irradiation. The free aminooxy groups so obtained are subsequently reacted with aldehyde-containing oligonucleotides to achieve efficient surface patterning.

• 出版日期2008-4-15
• 单位中国地震局