Detection of Antibodies to the Biofilm Exopolysaccharide of Histophilus somni following Infection in Cattle by Enzyme-Linked Immunosorbent Assay

作者：Pan Yu; Fisher Taylor; Olk Christina; Inzana Thomas J^*

来源：Clinical and Vaccine Immunology, 2014, 21(10): 1463-1467.

DOI：10.1128/CVI.00384-14

An enzyme-linked immunosorbent assay (ELISA) was developed to detect bovine antibodies to Histophilus somni exopolysaccharide (EPS), which is created during biofilm formation. When an index value of 0.268 was used, the sensitivity of the assay for infected calves was 90.5% at 3 weeks postinfection, but the number of positive animals increased by week 4. The specificity of the assay for healthy calves was 92.5%. The EPS ELISA may aid in identifying calves with H. somni diseases.

• 出版日期2014-10
• 单位美国弗吉尼亚理工大学(Virginia Tech)