Analysis of CTLA-4+49A/G gene polymorphism in cases with leprosy of Azerbaijan, Northwest Iran

作者：Almasi Shohreh; Aliparasti Mohammad Reza; Naghili Behrouz; Yeganeh Khalil; Rahnama Badrossadat; Tavanafar Fatemeh; Karzar Bita Hazhir; Khiabani Syamak Amini; Naghili Arman; Babaloo Zohreh^*

来源：Infection, Genetics and Evolution, 2018, 57: 121-127.

DOI：10.1016/j.meegid.2017.11.001

• Leprosy
• CTLA-4
• Gene polymorphism
• T-ARMS-PCR
• Age at the disease onset
• Performance status

Leprosy, which is developed by the obligate intracellular Mycobacterium leprae (ML); has different manifestations, associated with the host immune responses. The protective immune response against ML includes T-cell-mediated immunity. The CTLA-4 has a great impact as a negative regulator of the immune response and maintenance of peripheral tolerance.
This study analyzed the relationship between CTLA-4 + 49A/G gene polymorphism and clinical manifestation of leprosy disease and susceptibility among the Azeri population living Northwest Iran.
One hundred and ninety-two leprosy patients and 185 healthy controls participated in the study. CTLA-4 + 49A/G genotyping was conducted via tetra-primer amplification refractory mutation system-polymerase chain reaction (T-ARMS-PCR) analysis.
The allelic and genotypic frequencies of + 49A/G gene polymorphism were similar in controls and patients. However, older ages, older age of onset and over-representation in male were observed in lepromatous leprosy patient carriers of GG genotype. The current study demonstrates that although CTLA-4 + 49A/G polymorphism was not correlated with a higher genetic risk for leprosy, the presence of a GG genotype was associated with older ages, older age of onset and over-representation in male in Iranian Azeri population.

• 出版日期2018-1