A Versatile Liquid Chromatographic Technique for Pharmacokinetic Estimation of Curcumin in Human Plasma

作者：Gugulothu Dalapathi; Desai Preshita; Patravale Vandana^*

来源：Journal of Chromatographic Science, 2014, 52(8): 872-879.

DOI：10.1093/chromsci/bmt131

A simple, rapid, sensitive and specific liquid chromatographic method was developed and validated for the determination of curcumin in human plasma. Berberine was used as the internal standard. Chromatographic separation was achieved on a Zorbax Eclipse C-18 column at 40 degrees C, with a mobile phase consisting of 1% acetic acid (pH 3 adjusted with 50% triethanolamine): acetonitrile (55:45), at a flow rate of 1.25 mL/min. The method was validated for precision, accuracy, linearity, lower limit of quantification (LLOQ) and extraction efficiency according to the International Conference on Harmonization guidelines. The method was successfully developed with an LLOQ of 10 ng/mL and a runtime of 9 min. Linearity range was from 10 to 1000 ng/mL. Curcumin and Berberine were well separated with retention times of 8.2 +/- 0.2 and 1.4 +/- 0.1 min, respectively. Further, the method was successfully employed to study the pharmacokinetic parameters of curcumin, following oral administration of curcumin-loaded hydroxy propyl cellulose (HPC) nanoparticles and curcumin suspension in female Wistar rats. Curcumin-loaded HPC nanoparticles (C-max: 106.01 +/- 20.11 ng/mL) showed significant improvement in pharmacokinetic parameters when compared with curcumin suspension (C-max: 30.13 +/- 0.47 ng/mL) indicating 43.73-fold increase in relative bioavailability.

• 出版日期2014-9